Monday, May 27, 2024


Table of Contents


  1. A tray with the following:
    • Sterile syringes and needles
    • Antimicrobial solution
    • Appropriate specimen bottle
    • Dry cotton wool swabs in a gallipot
    • Disposable gloves
    • Tourniquet
    • Adhesive strip
    • Receiver for used items
    • Appropriate laboratory or specimen form
    • Appropriate PPE’s e.g. apron
    • Sharps container


  1. Establish rapport (Refer to steps)
  2. Explain procedure to the patient (Refer to steps)
  3. Perform hand hygiene
  4. Take tray and send to the bedside
  5. Provide privacy
  6. Protect the bed with a mackintosh and a dressing towel
  7. Assist patient into a desirable position
  8. Assist the doctor or laboratory technician to take the sample
  9. Dispose off used items and decontaminate the tray
  10. Ensure all needles are disposed off in the sharps container
  11. Ensure labelled specimen with signed laboratory forms are sent to the laboratory
  12. If the nurse is taking the blood specimen then the following steps should be followed after step 7
  13. Choose a site preferably on the forearm
  14. Apply tourniquet to locate a prominent vein
  15. Clean the site with antimicrobial solution
  16. Insert the needle gently with syringe attached into the vein at an angle of 45
  17. Withdraw the required amount of blood
  18. Release the tourniquet
  19. Remove the needle gently from the vein, apply a dry swab to the area immediately and hold it in place with an adhesive strip
  20. Disconnect needle from syringe, poor blood into specimen bottle
  21. Ensure specimen container is well labelled
  22. Perform hand hygiene